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mouse anti fasciclin iii  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse anti fasciclin iii
    Mouse Anti Fasciclin Iii, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+fasciclin+iii/anti-Fasciclin+III/bio_rxiv__64898__2026__04__14__718603-231-18-23
    Average 97 stars, based on 573 article reviews
    mouse anti fasciclin iii - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: The people behind the papers - Tiffany Roach and Kari Lenhart.
    Article Snippet: Primary antibodies used were: rat anti-DE-cadherin [Developmental Studies Hybridoma Bank (DSHB), 1:20], mouse anti-1B1 (DSHB, 1:50), chick anti-GFP (Aves Labs, 1020, 1:1000), guinea pig anti-traffic jam (Dorothea Godt, University of Toronto, Canada, 1:5000), mouse anti-fasciclin III (DSHB, 1:50), rabbit anti-phospho-p44/42MAPK Erk (1/2) (Cell Signaling Technology, 9101, 1:100) and mouse anti-broad-core (25E9.D7)-s (DSHB, 1:50).

    Article Title: TET exhibits enzymatic-independent and-dependent functions during Drosophila flight muscle development and aging
    Article Snippet: The following antibodies were used: goat anti-GFP (Abcam ab6673, 1/500), rabbit anti-GFP (Abcam ab6556, 1/500), mouse anti-Fasciclin III (DSHB 7G10, 1/25), rat anti-Kettin (Abcam ab50585, 1/200), mouse anti-Cut (DSHB 2B10, 1/100), rabbit anti-Zfh1 (a kind gift from R. Lehmann, 1/500), rabbit anti-Mef2 (a kind gift from E. Furlong, 1/500), rabbit anti-Twi (a kind gift from K. Jagla, 1/600), mouse anti-Mhc (DSHB 3E8-3D3, 1/50), mouse anti-ATP synthase subunit α (Abcam ab14748, 1/200), mouse anti-α-actinin (DSHB 2G3-3D7, 1/20), rat anti-Elav (DSHB 7E8A10, 1/100) rabbit anti-cleaved Dcp-1 (Cell Signaling Technology #9578, 1/500), mouse anti-Histone H3 phosphoSerine 10 (Abcam #14,955, 1/1000), anti-polyUbiquitin (Sigma-Aldrich FK2, 1/200), donkey anti-rabbit, anti-mouse or anti-goat Alexa Fluor 488 (all from Invitrogen, 1/300), donkey anti-rat or anti-mouse Cy3/Cy5 (Jackson ImmunoResearch, 1/300).

    Article Title: Tbx1 ortholog org-1 is required to establish testis stem cell niche identity in Drosophila
    Article Snippet: We used goat anti Vasa 1:800 (Santa Cruz Biotech -Discont.); rabbit anti Vasa 1:5000 (BosterBio DZ41154); mouse anti Fasciclin III 1:50 (DSHB AB_528238); rat anti N-cadherin 1:20 (DSHB AB_528121); rat anti E-cad 1:20 (DSHB AB_528120); rabbit anti Stat92E 1:200 (gift from E. Bach, NYU); rabbit anti RFP 1:500 (Abcam ab62341); mouse anti Islet 1:200 (DSHB AB_528313); guinea pig anti Traffic jam 1:10,000 (Gift from D. Godt); rat anti Org-1 1:200 (gift from C. Schaub); mouse anti Gamma Tubulin 1:200 (Sigma AB_477584); chicken anti Beta-gal 1:1000 (Abcam ab9361-250); mouse anti Patched 1:50 (DSHB AB_528441); and chicken anti GFP 1:1000 (Aves labs AB_2307313).

    Article Title: Identification of loop regions as motifs determining cellular and organ chirality in Myosin 1C
    Article Snippet: The following primary antibodies were used at the dilution indicated: rabbit anti-RFP (1:1,000, MBL), rabbit anti-GFP (1:1000, MBL), mouse anti-Fasciclin III (1:100, 7G10, Developmental Studies Hybridoma Bank), and chicken anti-β-galactosidase (1:500, Abcam).

    Article Title: TET exhibits enzymatic-independent and-dependent functions during Drosophila flight muscle development and aging.
    Article Snippet: The following antibodies were used: goat anti-GFP (Abcam ab6673, 1/500), rabbit anti-GFP (Abcam ab6556, 1/500), mouse anti-Fasciclin III (DSHB 7G10, 1/25), rat anti-Kettin (Abcam ab50585, 1/200), mouse anti-Cut (DSHB 2B10, 1/100), rabbit anti-Zfh1 (a kind gift from R. Lehmann, 1/500), rabbit anti-Mef2 (a kind gift from E. Furlong, 1/500), rabbit anti-Twi (a kind gift from K. Jagla, 1/600), mouse anti-Mhc (DSHB 3E8-3D3, 1/50), mouse anti-ATP synthase subunit α (Abcam ab14748, 1/200), mouse anti-α-actinin (DSHB 2G3-3D7, 1/20), rat antiElav (DSHB 7E8A10, 1/100) rabbit anti-cleaved Dcp-1 (Cell Signaling Technology #9578, 1/500), mouse antiHistone H3 phosphoSerine 10 (Abcam #14,955, 1/1000), anti-polyUbiquitin (Sigma-Aldrich FK2, 1/200), donkey anti-rabbit, anti-mouse or anti-goat Alexa Fluor 488 (all from Invitrogen, 1/300), donkey anti-rat or anti-mouse Cy3/Cy5 (Jackson ImmunoResearch, 1/300).

    Article Title: Functional Analysis of Forkhead Transcription Factor Fd59a in the Spermatogenesis of Drosophila melanogaster
    Article Snippet: The primary antibodies used in this study were as follows: rat anti-Vasa (1:50, Developmental Studies Hybridoma Bank, Iowa, IA, USA), mouse anti-Fasciclin III (Fas III) (1:100, Developmental Studies Hybridoma Bank, 7G10, Iowa, IA, USA), and mouse anti-αSpectrin (1:50, Developmental Studies Hybridoma Bank, 3A9, Iowa, IA, USA).

    Incubation:

    Article Title: The Tbx1 ortholog org-1 is required to establish testis stem cell niche identity in Drosophila
    Article Snippet: .. Dissected tissue was incubated at 4°C overnight with primary antibodies as follows: goat anti-Vasa [1:800; Santa Cruz Biotech (discontinued)], rabbit anti-Vasa (1:5000; BosterBio, DZ41154), mouse anti-Fasciclin III (1:50; DSHB AB_528238), rat anti-N-cadherin (1:20; DSHB AB_528121), rat anti-E-cad (1:20; DSHB AB_528120), rabbit anti-Stat92E (1:200; ), rabbit anti-RFP (1:500; Abcam ab62341), mouse anti-Islet (1:200; DSHB AB_528313), guinea pig anti-Traffic jam (1:10,000; a gift from D. Godt, University of Toronto, Canada), rat anti Org-1 (1:200; a gift from C. Schaub, EMBL, Heidelberg, Germany), mouse anti-Gamma Tubulin (1:200; Sigma AB_477584), chicken anti-Beta-gal (1:1000; Abcam ab9361-250), mouse anti-Patched (1:50; DSHB AB_528441) and chicken anti-GFP (1:1000; Aves labs AB_2307313). .. Secondary antibodies were used at 3.75 μg/ml (Alexa488, Cy3 or Alexa647; Molecular Probes; Jackson Immunoresearch) for 1.5-2 h at room temperature.

    Concentration Assay:

    Article Title: Sec61β, a subunit of the Sec61 complex at the endoplasmic reticulum, coordinates with Ocnus in regulating Drosophila spermatogenesis.
    Article Snippet: sec61β encodes a subunit of the Sec61 translocon which is a highly conserved heterotrimer responsible for translocating the nascent polypeptides into the lumen of the endoplasmic reticulum (ER) or onto the ER membrane.. In this study, we show that knockdown of sec61β in the early germline leads to male sterility in Drosophila melanogaster.. These males exhibit testes that are dramatically reduced in size and devoid of germ cells.



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    A The table shows the division orientation of dedifferentiated cells after reattachment to the hub in the steady-state conditions (upper table) (listed in Table ) and during the recovery period (lower table) after partial depletion of GSCs (listed in Table ). Dedifferentiated cells that did not divide after reattachment are not shown. Orientation was judged as “oriented” when one of the two spindle poles was closely associated with the hub during mitosis. B Schematic of centrosome orientation checkpoint (COC). When centrosomes are positioned perpendicularly towards the hub-GSC interface, COC is cleared and GSCs are allowed to enter mitosis. When centrosomes are mispositioned, COC blocks GSCs from progression to mitotic entry and GSCs are arrested in the late G2 phase. C , D Representative immunofluorescence images of Wild type ( yw , 6 x heat-shock treated and recovered for 3 days) and hs-bam (6 x heat-shock treated and recovered for 3 days) following 4.5 h of ex-vivo colcemid treatment. The testes were stained with phospho-histone-3 (PH3, red), Vasa (blue), <t>FasIII</t> (green), and Hts (green) antibodies. CySC=cyst stem cells. E , F Box plots show 25–75% (box), minimum to maximum (whiskers) with all data points. In E , each data point indicates percentage of the PH3 positive cells in scored testis after 4.5-h colcemid treatment. “n” indicates the number of scored testes. In F , changes in GSC number after forced differentiation of GSCs are shown. From pre-heat shock (pre-hs), post-heat shock (post-hs), to 3- and 5-day recovery points (3 d rec, 5 d rec) without or with expression of Baz RNAi are shown. nosGal4 driver was used to express RNAi construct against Baz. “n” indicates the number of scored testes. Šídák’s multiple tests calculated p -values in E , F . G A representative immunofluorescence image of a Baz-GFP Flytrap testis with centrosome staining (anti-γTub, or GTU-88). FasIII and γTub (red), Vasa (blue), Baz-GFP (green). Asterisks in all images indicate the approximate location of the hub. All scale bars represent 10 μm.
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    A The table shows the division orientation of dedifferentiated cells after reattachment to the hub in the steady-state conditions (upper table) (listed in Table ) and during the recovery period (lower table) after partial depletion of GSCs (listed in Table ). Dedifferentiated cells that did not divide after reattachment are not shown. Orientation was judged as “oriented” when one of the two spindle poles was closely associated with the hub during mitosis. B Schematic of centrosome orientation checkpoint (COC). When centrosomes are positioned perpendicularly towards the hub-GSC interface, COC is cleared and GSCs are allowed to enter mitosis. When centrosomes are mispositioned, COC blocks GSCs from progression to mitotic entry and GSCs are arrested in the late G2 phase. C , D Representative immunofluorescence images of Wild type ( yw , 6 x heat-shock treated and recovered for 3 days) and hs-bam (6 x heat-shock treated and recovered for 3 days) following 4.5 h of ex-vivo colcemid treatment. The testes were stained with phospho-histone-3 (PH3, red), Vasa (blue), <t>FasIII</t> (green), and Hts (green) antibodies. CySC=cyst stem cells. E , F Box plots show 25–75% (box), minimum to maximum (whiskers) with all data points. In E , each data point indicates percentage of the PH3 positive cells in scored testis after 4.5-h colcemid treatment. “n” indicates the number of scored testes. In F , changes in GSC number after forced differentiation of GSCs are shown. From pre-heat shock (pre-hs), post-heat shock (post-hs), to 3- and 5-day recovery points (3 d rec, 5 d rec) without or with expression of Baz RNAi are shown. nosGal4 driver was used to express RNAi construct against Baz. “n” indicates the number of scored testes. Šídák’s multiple tests calculated p -values in E , F . G A representative immunofluorescence image of a Baz-GFP Flytrap testis with centrosome staining (anti-γTub, or GTU-88). FasIII and γTub (red), Vasa (blue), Baz-GFP (green). Asterisks in all images indicate the approximate location of the hub. All scale bars represent 10 μm.
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    Image Search Results


    A The table shows the division orientation of dedifferentiated cells after reattachment to the hub in the steady-state conditions (upper table) (listed in Table ) and during the recovery period (lower table) after partial depletion of GSCs (listed in Table ). Dedifferentiated cells that did not divide after reattachment are not shown. Orientation was judged as “oriented” when one of the two spindle poles was closely associated with the hub during mitosis. B Schematic of centrosome orientation checkpoint (COC). When centrosomes are positioned perpendicularly towards the hub-GSC interface, COC is cleared and GSCs are allowed to enter mitosis. When centrosomes are mispositioned, COC blocks GSCs from progression to mitotic entry and GSCs are arrested in the late G2 phase. C , D Representative immunofluorescence images of Wild type ( yw , 6 x heat-shock treated and recovered for 3 days) and hs-bam (6 x heat-shock treated and recovered for 3 days) following 4.5 h of ex-vivo colcemid treatment. The testes were stained with phospho-histone-3 (PH3, red), Vasa (blue), FasIII (green), and Hts (green) antibodies. CySC=cyst stem cells. E , F Box plots show 25–75% (box), minimum to maximum (whiskers) with all data points. In E , each data point indicates percentage of the PH3 positive cells in scored testis after 4.5-h colcemid treatment. “n” indicates the number of scored testes. In F , changes in GSC number after forced differentiation of GSCs are shown. From pre-heat shock (pre-hs), post-heat shock (post-hs), to 3- and 5-day recovery points (3 d rec, 5 d rec) without or with expression of Baz RNAi are shown. nosGal4 driver was used to express RNAi construct against Baz. “n” indicates the number of scored testes. Šídák’s multiple tests calculated p -values in E , F . G A representative immunofluorescence image of a Baz-GFP Flytrap testis with centrosome staining (anti-γTub, or GTU-88). FasIII and γTub (red), Vasa (blue), Baz-GFP (green). Asterisks in all images indicate the approximate location of the hub. All scale bars represent 10 μm.

    Journal: Communications Biology

    Article Title: Stem cells resume asymmetric division upon niche re-entry through reactivating the centrosome orientation checkpoint

    doi: 10.1038/s42003-026-09812-7

    Figure Lengend Snippet: A The table shows the division orientation of dedifferentiated cells after reattachment to the hub in the steady-state conditions (upper table) (listed in Table ) and during the recovery period (lower table) after partial depletion of GSCs (listed in Table ). Dedifferentiated cells that did not divide after reattachment are not shown. Orientation was judged as “oriented” when one of the two spindle poles was closely associated with the hub during mitosis. B Schematic of centrosome orientation checkpoint (COC). When centrosomes are positioned perpendicularly towards the hub-GSC interface, COC is cleared and GSCs are allowed to enter mitosis. When centrosomes are mispositioned, COC blocks GSCs from progression to mitotic entry and GSCs are arrested in the late G2 phase. C , D Representative immunofluorescence images of Wild type ( yw , 6 x heat-shock treated and recovered for 3 days) and hs-bam (6 x heat-shock treated and recovered for 3 days) following 4.5 h of ex-vivo colcemid treatment. The testes were stained with phospho-histone-3 (PH3, red), Vasa (blue), FasIII (green), and Hts (green) antibodies. CySC=cyst stem cells. E , F Box plots show 25–75% (box), minimum to maximum (whiskers) with all data points. In E , each data point indicates percentage of the PH3 positive cells in scored testis after 4.5-h colcemid treatment. “n” indicates the number of scored testes. In F , changes in GSC number after forced differentiation of GSCs are shown. From pre-heat shock (pre-hs), post-heat shock (post-hs), to 3- and 5-day recovery points (3 d rec, 5 d rec) without or with expression of Baz RNAi are shown. nosGal4 driver was used to express RNAi construct against Baz. “n” indicates the number of scored testes. Šídák’s multiple tests calculated p -values in E , F . G A representative immunofluorescence image of a Baz-GFP Flytrap testis with centrosome staining (anti-γTub, or GTU-88). FasIII and γTub (red), Vasa (blue), Baz-GFP (green). Asterisks in all images indicate the approximate location of the hub. All scale bars represent 10 μm.

    Article Snippet: The primary antibodies used were as follows: rat anti-Vasa (1:20; developed by A. Spradling and D. Williams, obtained from Developmental Studies Hybridoma Bank (DSHB); mouse anti-hu-li tai shao (Hts) (1:20, 1B1; DSHB); mouse-anti-Fasciclin III (FasIII) (1:40, 7G10; DSHB); mouse anti-γ-Tubulin (1:400, GTU-88; Sigma-Aldrich); rabbit anti-phosphorylated (Thr3) histone H3 (1:200; clone JY325, Upstate).

    Techniques: Immunofluorescence, Ex Vivo, Staining, Expressing, Construct